听力与言语-语言病理学

行为科学

医学伦理学

你正在浏览AMERICAN JOURNAL OF BOTANY期刊下所有文献
  • Plastid genomes reveal recurrent formation of allopolyploid Fragaria.

    abstract:PREMISE OF THE STUDY:Recurrent formation of polyploid taxa is a common observation in many plant groups. Haploid, cytoplasmic genomes like the plastid genome can be used to overcome the problem of homeologous genes and recombination in polyploid taxa. Fragaria (Rosaceae) contains several octo- and decaploid species. We...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1085

    authors: Dillenberger MS,Wei N,Tennessen JA,Ashman TL,Liston A

    更新日期:2018-05-01 00:00:00

  • C4 photosynthesis and transition of Kranz anatomy in cotyledons and leaves of Tetraena simplex.

    abstract:PREMISE OF THE STUDY:Tetraena simplex is an independently evolved C4 species in the Zygophylloideae (Zygophyllaceae) and a characteristic forb of saline flats in hot and sandy desert habitats. During early ontogeny, the species had a morphological shift from planar cotyledons (dorsiventral symmetry) to terete, succulen...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1087

    authors: Muhaidat R,McKown AD,Al Zoubi M,Bani Domi Z,Otoum O

    更新日期:2018-05-01 00:00:00

  • Transgenerational epigenetics: Inheritance of global cytosine methylation and methylation-related epigenetic markers in the shrub Lavandula latifolia.

    abstract:PREMISE OF THE STUDY:The ecological and evolutionary significance of natural epigenetic variation (i.e., not based on DNA sequence variants) variation will depend critically on whether epigenetic states are transmitted from parents to offspring, but little is known on epigenetic inheritance in nonmodel plants. METHODS...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1074

    authors: Herrera CM,Alonso C,Medrano M,Pérez R,Bazaga P

    更新日期:2018-04-01 00:00:00

  • The role of diversification in community assembly of the oaks (Quercus L.) across the continental U.S.

    abstract:PREMISE OF THE STUDY:Evolutionary and biogeographic history, including past environmental change and diversification processes, are likely to have influenced the expansion, migration, and extinction of populations, creating evolutionary legacy effects that influence regional species pools and the composition of communi...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1049

    authors: Cavender-Bares J,Kothari S,Meireles JE,Kaproth MA,Manos PS,Hipp AL

    更新日期:2018-03-01 00:00:00

  • Next-generation lineage discovery: A case study of tuberous Claytonia L.

    abstract:PREMISE OF THE STUDY:Species formation is an intuitive endpoint of reproductive isolation, but circumscribing taxa that arise during speciation can be difficult because of gene flow, morphological continuity, hybridization or polyploidization, and low sequence variation among newly diverged lineages. Nonetheless, speci...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1061

    authors: Stoughton TR,Kriebel R,Jolles DD,O'Quinn RL

    更新日期:2018-03-01 00:00:00

  • Community assembly of the ferns of Florida.

    abstract:PREMISE OF THE STUDY:Many ecological and evolutionary processes shape the assembly of organisms into local communities from a regional pool of species. We analyzed phylogenetic and functional diversity to understand community assembly of the ferns of Florida at two spatial scales. METHODS:We built a phylogeny for 125 ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1073

    authors: Sessa EB,Chambers SM,Li D,Trotta L,Endara L,Burleigh JG,Baiser B

    更新日期:2018-03-01 00:00:00

  • Constructing a broadly inclusive seed plant phylogeny.

    abstract:PREMISE OF THE STUDY:Large phylogenies can help shed light on macroevolutionary patterns that inform our understanding of fundamental processes that shape the tree of life. These phylogenies also serve as tools that facilitate other systematic, evolutionary, and ecological analyses. Here we combine genetic data from pu...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1019

    authors: Smith SA,Brown JW

    更新日期:2018-03-01 00:00:00

  • Limited pollen dispersal, small genetic neighborhoods, and biparental inbreeding in Vallisneria americana.

    abstract:PREMISE OF THE STUDY:Pollen dispersal is a key process that influences ecological and evolutionary dynamics of plant populations by facilitating sexual reproduction and gene flow. Habitat loss and fragmentation have the potential to reduce pollen dispersal within and among habitat patches. We assessed aquatic pollen di...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1031

    authors: Lloyd MW,Tumas HR,Neel MC

    更新日期:2018-02-01 00:00:00

  • Greatly reduced phylogenetic structure in the cultivated potato clade (Solanum section Petota pro parte).

    abstract:PREMISE OF THE STUDY:The species boundaries of wild and cultivated potatoes are controversial, with most of the taxonomic problems in the cultivated potato clade. We here provide the first in-depth phylogenetic study of the cultivated potato clade to explore possible causes of these problems. METHODS:We examined 131 d...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1008

    authors: Spooner DM,Ruess H,Arbizu CI,Rodríguez F,Solís-Lemus C

    更新日期:2018-01-01 00:00:00

  • Dynamics of gaps, vegetation, and plant species with and without fire.

    abstract:PREMISE OF THE STUDY:Areas lacking dominant plants, or gaps, can support high diversity and specialist species. Previous chronosequence research in Florida rosemary scrub showed indistinct gap area patterns with fire and the dependence of certain species on gaps. We hypothesized that fire and gap size would affect exti...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1700175

    authors: Menges ES,Crate SJH,Quintana-Ascencio PF

    更新日期:2017-12-01 00:00:00

  • Complete chloroplast genome sequences contribute to plant species delimitation: A case study of the Anemopaegma species complex.

    abstract:PREMISE OF THE STUDY:Bignoniaceae is an important component of neotropical forests and a model for evolutionary and biogeographical studies. A previous combination of molecular markers and morphological traits improved the phylogeny of the group. Here we demonstrate the value of next-generation sequencing (NGS) to asse...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1700302

    authors: Firetti F,Zuntini AR,Gaiarsa JW,Oliveira RS,Lohmann LG,Van Sluys MA

    更新日期:2017-10-01 00:00:00

  • MicroCT imaging as a tool to study vessel endings in situ.

    abstract:PREMISE OF THE STUDY:Despite the strong influence of the frequency and distribution of vessel endings on both hydraulic safety and efficiency, detailed anatomical descriptions or measurements of these structures are generally lacking. METHODS:Here we used high-resolution x-ray microcomputed tomography (microCT) to ide...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1700199

    authors: Wason JW,Huggett BA,Brodersen CR

    更新日期:2017-09-01 00:00:00

  • Learning in two butterfly species when using flowers of the tropical milkweed Asclepias curassavica: No benefits for pollination.

    abstract:PREMISE OF THE STUDY:The ability of insect visitors to learn to manipulate complex flowers has important consequences for foraging efficiency and plant fitness. We investigated learning by two butterfly species, Danaus erippus and Heliconius erato, as they foraged on the complex flowers of Asclepias curassavica, as wel...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1700040

    authors: Ramos BCM,Rodríguez-Gironés MA,Rodrigues D

    更新日期:2017-08-01 00:00:00

  • High growth temperatures and high soil nitrogen do not alter differences in CO2 assimilation between invasive Phalaris arundinacea (reed canarygrass) and Carex stricta (tussock sedge).

    abstract:PREMISE OF THE STUDY:Global change in temperature and soil nitrogen availability could affect plant community composition, potentially giving an advantage to invasive species compared to native species. We addressed how high temperatures affected CO2 assimilation parameters for invasive Phalaris arundinacea and a sedge...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600339

    authors: Waring EF,Holaday AS

    更新日期:2017-07-01 00:00:00

  • Common garden test of range limits as predicted by a species distribution model in the annual plant Mimulus bicolor.

    abstract:PREMISE OF THE STUDY:Direct tests of a species distribution model (SDM) were used to evaluate the hypothesis that the northern and southern edges of Mimulus bicolor's geographical range are limited by temperature and precipitation. METHODS:Climatic suitability was predicted using an SDM informed only by temperature an...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600414

    authors: Dixon AL,Busch JW

    更新日期:2017-06-01 00:00:00

  • Pollinia transfer on moth legs in Hoya carnosa (Apocynaceae).

    abstract:PREMISE OF THE STUDY:The genus Hoya (Apocynaceae; Asclepiadoideae) is characterized by a set of complex floral characters unique among the asclepiads, but their role in pollination is poorly understood. Here, we report a new mechanism of asclepiad pollination in the wax plant Hoya carnosa: the pollinaria are transferre...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1700078

    authors: Mochizuki K,Furukawa S,Kawakita A

    更新日期:2017-06-01 00:00:00

  • Carbon allocation to growth and storage in two evergreen species of contrasting successional status.

    abstract:PREMISE OF THE STUDY:A prevailing hypothesis in forest succession is that shade-tolerant species grow more slowly than shade-intolerant species, across light conditions, because they prioritize carbon (C) allocation to storage. We examined this hypothesis in a confamilial pair of species, including one of the fastest-g...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1700057

    authors: Piper FI,Sepúlveda P,Bustos-Salazar A,Zúñiga-Feest A

    更新日期:2017-05-01 00:00:00

  • Photoperiod throughout the maternal life cycle, not photoperiod during seed imbibition, influences germination in Arabidopsis thaliana.

    abstract:PREMISE OF THE STUDY:Plants adjust their phenology in response to seasonal cues experienced both by their parents and by themselves, and coordinating responses to these cues is necessary for expressing adaptive phenology. We investigated how cues are integrated across time to influence an important progeny phenotype, i...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600389

    authors: Imaizumi T,Auge G,Donohue K

    更新日期:2017-04-01 00:00:00

  • The evolution of aluminum accumulation in ferns and lycophytes.

    abstract:PREMISE OF THE STUDY:This paper investigates the occurrence and evolution of aluminum (Al) accumulation within ferns and lycophytes, which is characterized by Al concentrations above 1000 mg·kg-1 in aboveground plant tissues. We hypothesize that this feature is more common in ferns than in angiosperms, and potentially ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600381

    authors: Schmitt M,Mehltreter K,Sundue M,Testo W,Watanabe T,Jansen S

    更新日期:2017-04-01 00:00:00

  • Into Africa: Molecular phylogenetics and historical biogeography of sub-Saharan African woodferns (Dryopteris).

    abstract:PREMISE OF THE STUDY:Our goal was to infer the phylogenetic relationships and historical biogeography of the genus Dryopteris with a focus on taxa in sub-Saharan Africa and neighboring islands. In general, little is known about the relationships between African fern species and their congeners in other geographic regio...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600392

    authors: Sessa EB,Juslén A,Väre H,Chambers SM

    更新日期:2017-03-01 00:00:00

  • Flexibility of resource allocation in a hermaphroditic-gynomonoecious herb through deployment of female and male resources in perfect flowers.

    abstract:PREMISE OF THE STUDY:It has been hypothesized that two flower types permit flexible allocation of resources to female and male functions, yet empirical evidence for the sex-allocation hypothesis remains scarce in gynomonoecious species. To characterize resource allocation to pistillate and perfect flowers and allocatio...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600397

    authors: Mamut J,Xiong YZ,Tan DY,Huang SQ

    更新日期:2017-03-01 00:00:00

  • Associations between sex-organ deployment and morph bias in related heterostylous taxa with different stylar polymorphisms.

    abstract:PREMISE OF THE STUDY:Populations of heterostylous species are characterized by two or three floral morphs with reciprocal positioning of stigmas and anthers. Theoretical models predict equal morph frequencies (isoplethy) when disassortative mating is prevalent in populations, but biased morph ratios may occur when vari...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600345

    authors: Ferrero V,Barrett SC,Rojas D,Arroyo J,Navarro L

    更新日期:2017-01-01 00:00:00

  • Germination responses to current and future temperatures of four seeder shrubs across a latitudinal gradient in western Iberia.

    abstract:PREMISE OF THE STUDY:Species differ in their temperature germination niche. Populations of a species may similarly differ across the distribution range of the species. Anticipating the impacts of climate variability and change requires understanding the differential sensitivity to germination temperature among and with...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600278

    authors: Chamorro D,Luna B,Moreno JM

    更新日期:2017-01-01 00:00:00

  • Related allopolyploids display distinct floral pigment profiles and transgressive pigments.

    abstract:PREMISE OF THE STUDY:Both polyploidy and shifts in floral color have marked angiosperm evolution. Here, we investigate the biochemical basis of the novel and diverse floral phenotypes seen in allopolyploids in Nicotiana (Solanaceae) and examine the extent to which the merging of distinct genomes alters flavonoid pigmen...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600350

    authors: McCarthy EW,Berardi AE,Smith SD,Litt A

    更新日期:2017-01-01 00:00:00

  • A subgeneric classification of Selaginella (Selaginellaceae).

    abstract:PREMISE OF THE STUDY:The lycophyte family Selaginellaceae includes approximately 750 herbaceous species worldwide, with the main species richness in the tropics and subtropics. We recently presented a phylogenetic analysis of Selaginellaceae based on DNA sequence data and, with the phylogeny as a framework, the study d...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600288

    authors: Weststrand S,Korall P

    更新日期:2016-12-01 00:00:00

  • Functional trait values, not trait plasticity, drive the invasiveness of Rosa sp. in response to light availability.

    abstract:PREMISE OF THE STUDY:Functional trait plasticity in resource capture traits has been suggested as an underlying mechanism promoting invasive species establishment and spread. Earlier studies on this mechanism treat invasiveness as a discrete characteristic (i.e., invasive vs. noninvasive) and do not consider the potent...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600235

    authors: Murphy JE,Burns JH,Fougère-Danezan M,Drenovsky RE

    更新日期:2016-12-01 00:00:00

  • Land-use change has no detectable effect on reproduction of a disturbance-adapted, hawkmoth-pollinated plant species.

    abstract:PREMISE OF THE STUDY:Land-use change is cited as a primary driver of global biodiversity loss, with myriad consequences for species, populations, and ecosystems. However, few studies have examined its impact on species interactions, particularly pollination. Furthermore, when the effects of land-use change on pollinati...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600302

    authors: Skogen KA,Jogesh T,Hilpman ET,Todd SL,Rhodes MK,Still SM,Fant JB

    更新日期:2016-11-01 00:00:00

  • Light availability affects sex lability in a gynodioecious plant.

    abstract:PREMISE OF THE STUDY:Sex lability (i.e., gender diphasy) in plants is classically linked to the larger resource needs associated with the female sexual function (i.e., seed production) compared to the male function (i.e., pollen production). Sex lability in response to the environment is extensively documented in dioec...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600158

    authors: Varga S,Kytöviita MM

    更新日期:2016-11-01 00:00:00

  • Inferences of population structure and demographic history for Taxodium distichum, a coniferous tree in North America, based on amplicon sequencing analysis.

    abstract:PREMISE OF THE STUDY:Studies of natural genetic variation can elucidate the genetic basis of phenotypic variation and the past population structure of species. Our study species, Taxodium distichum, is a unique conifer that inhabits the flood plains and swamps of North America. Morphological and ecological differences ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600046

    authors: Ikezaki Y,Suyama Y,Middleton BA,Tsumura Y,Teshima K,Tachida H,Kusumi J

    更新日期:2016-11-01 00:00:00

  • Mutations in two pollen self-incompatibility factors in geographically marginal populations of Solanum habrochaites impact mating system transitions and reproductive isolation.

    abstract:PREMISE OF THE STUDY:Self-incompatibility (SI) is a mechanism that prevents inbreeding in many plant species. The mutational breakdown of SI occurs frequently, yet relatively little is known about the evolutionary steps involved in the progressive loss of pistil and pollen SI function. METHODS:In Solanaceae, SI is the...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600208

    authors: Markova DN,Petersen JJ,Qin X,Short DR,Valle MJ,Tovar-Méndez A,McClure BA,Chetelat RT

    更新日期:2016-10-01 00:00:00

  • Effects of plant intraspecific diversity across three trophic levels: Underlying mechanisms and plant traits.

    abstract:PREMISE OF STUDY:Although there is increasing recognition of the effects of plant intraspecific diversity on consumers, the mechanisms by which such effects cascade-up to higher trophic levels remain elusive. METHODS:We evaluated the effects of plant (lima bean, Phaseolus lunatus) intraspecific diversity on a suite of...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600234

    authors: Abdala-Roberts L,Hernández-Cumplido J,Chel-Guerrero L,Betancur-Ancona D,Benrey B,Moreira X

    更新日期:2016-10-01 00:00:00

  • X-rays and virtual taphonomy resolve the first Cissus (Vitaceae) macrofossils from Africa as early-diverging members of the genus.

    abstract:PREMISE OF THE STUDY:Fossilized seeds similar to Cissus (Vitaceae) have been recognized from the Miocene of Kenya, though some were previously assigned to the Menispermaceae. We undertook a comparative survey of extant African Cissus seeds to identify the fossils and consider their implications for the evolution and bi...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600177

    authors: Adams NF,Collinson ME,Smith SY,Bamford MK,Forest F,Malakasi P,Marone F,Sykes D

    更新日期:2016-09-01 00:00:00

  • Stress-induced memory alters growth of clonal offspring of white clover (Trifolium repens).

    abstract:PREMISE OF THE STUDY:The phenotype of an individual can be modified by the environment experienced by its predecessors, a phenomenon called transgenerational or maternal effects. These effects are studied mostly across sexual generations and are thought to be mediated also by epigenetic variation. However, we do not kn...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1500526

    authors: González AP,Chrtek J,Dobrev PI,Dumalasová V,Fehrer J,Mráz P,Latzel V

    更新日期:2016-09-01 00:00:00

  • Influence of plant resistance traits in selectiveness and species strength in a tropical plant-herbivore network.

    abstract:PREMISE OF THE STUDY:Plant-herbivore networks are highly specialized in their interactions, yet they are highly variable with regard to the relative importance of specific host species for herbivores. How host species traits determine specialization and species strength in this antagonistic network is still an unanswer...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600045

    authors: López-Carretero A,Boege K,Díaz-Castelazo C,Domínguez Z,Rico-Gray V

    更新日期:2016-08-01 00:00:00

  • How diploidization turned a tetraploid into a pseudotriploid.

    abstract:PREMISE OF THE STUDY:Despite being highly fertile and occupying a large geographic region, the North American heartleaf bittercress (Cardamine cordifolia; Brassicaceae) has a puzzling triploid-like chromosome number (2n = 3x = 24). As most triploids are sterile, we embarked on a detailed analysis of the C. cordifolia g...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1500452

    authors: Mandáková T,Gloss AD,Whiteman NK,Lysak MA

    更新日期:2016-07-01 00:00:00

  • Pollen from Arabidopsis thaliana and other Brassicaceae are functionally omniaperturate.

    abstract:PREMISE OF THE STUDY:Most pollen walls are interrupted by apertures, thin areas providing access to stigmatic fluids and exit points for pollen tubes. Unexpectedly, pollen tubes of Arabidopsis thaliana are not obligated to pass through apertures and can instead take the shortest route into the stigma, passing directly ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600031

    authors: Edlund AF,Zheng Q,Lowe N,Kuseryk S,Ainsworth KL,Lyles RH,Sibener SJ,Preuss D

    更新日期:2016-06-01 00:00:00

  • Bidirectional anatomical effects in a mistletoe-host relationship: Psittacanthus schiedeanus mistletoe and its hosts Liquidambar styraciflua and Quercus germana.

    abstract:PREMISE OF THE STUDY:During the interactions between a parasitic plant and its host, the parasite affects its host morphologically, anatomically, and physiologically, yet there has been little focus on the effect of hosts on the parasite. Here, the functional interactions between the hemiparasitic mistletoe Psittacanth...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600166

    authors: Cocoletzi E,Angeles G,Ceccantini G,Patrón A,Ornelas JF

    更新日期:2016-06-01 00:00:00

  • Phylogenetic diversification of Early Cretaceous seed plants: The compound seed cone of Doylea tetrahedrasperma.

    abstract:PREMISE OF THE STUDY:Discovery of cupulate ovules of Doylea tetrahedrasperma within a compact, compound seed cone highlights the rich diversity of fructification morphologies, pollination biologies, postpollination enclosure of seeds, and systematic diversity of Early Cretaceous gymnosperms. METHODS:Specimens were stu...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600030

    authors: Rothwell GW,Stockey RA

    更新日期:2016-05-01 00:00:00

  • Are trait-scaling relationships invariant across contrasting elevations in the widely distributed treeline species Nothofagus pumilio?

    abstract:PREMISE OF THE STUDY:The study of scaling examines the relative dimensions of diverse organismal traits. Understanding whether global scaling patterns are paralleled within species is key to identify causal factors of universal scaling. I examined whether the foliage-stem (Corner's rules), the leaf size-number, and the...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1500439

    authors: Fajardo A

    更新日期:2016-05-01 00:00:00

  • Flower-visitor selection on floral integration in three contrasting populations of Lonicera implexa.

    abstract:PREMISE OF THE STUDY:Geographic differences in flower visitor assemblages might lead to among-population differences in the magnitude and pattern of floral integration. However, the role of current pollinator visitation in shaping the magnitude and pattern of floral trait correlations is still controversial. METHODS:W...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1500336

    authors: Lázaro A,Santamaría L

    更新日期:2016-02-01 00:00:00

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